Hans Johansson | General Manager
Sunresin
Hans J Johansson is General Manager for Sunresin Sweden based in Uppsala, Sweden. He has spent more than 35 years in the Biotech industry. Most of the time in research and development at Pharmacia (now Cytiva), and Purolite (now Ecolab) with a special focus on design and applications of industrial chromatography resins intended for antibody purification and other therapeutic proteins. He frequently publishes in scientific journals and is the holder of more than ten patents around resin design and large-scale protein purification. He is currently leading the research centre in Uppsala, focusing on development and applications of novel, agarose-based, chromatography resins for all types of biomolecule purification.
Appearances:
Festival of Biologics Day 2 @ 11:50
Designing Optimal Protein A Resins for the Downstream Processing of Antibodies
In this communication, we report on the engineering of recombinant Protein A (rProtein A) to enhance its key properties, particularly alkaline stability.
We focused on targeted modifications to the C domain of the native Protein A, evaluating these changes through the development of multimeric variants. Among the variants assessed, the selected one shows remarkable alkaline stability up to 1.5M NaOH.
This ligand was used for the preparation of two different rProtein A agarose resins.
One of them was designed to be cost-effective, highly stable showing extremely high DBC.
A second rProtein A agarose resin was developed to maintain high DBC and alkaline stability with improved pressure flow characteristics thanks to a specific and innovative agarose resin design.
This presentation will discuss critical performance parameters when designing new agarose resins. Development, characterization & biomolecule application data for two Protein A affinity resins and other functionalized resins will be discussed.
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