Cell Culture World Congress 24th May



Nobel Vale

Opening remarks from the chair

Next generation cellular engineering

David Bruhlmann

Modulation of glycosylation by media design

  • An overview of glycosylation methods
  • Mechanisms by which media design evolve glycosylation patterns
  • Benefits of glycosylation by media design
Susan Sharfstein

Breaking the mould with cellular engineering

  • Using cell engineering to replace animal-derived therapeutics
  • Metabolic engineering of CHO cells-a new paradigm in bioprocessing
  • The role of bioprocessing in controlling productivity and product quality for novel biopharmaceuticals
David Wood

A non-Fc parallel to Protein A: A universal platform for tagless and traceless capture of non-mAb targets

  • Development of a robust and reliable self-cleaving tag capture resin
  • Case studies and results for recent biosimilars
  • Comparisons to Protein A from a regulatory and process impact standpoint

Speed Networking



Quality by design

Scott Tenenbaum

Using non-coding RNAs for Cell Line Selection

  • Replace resistance genes with non-coding RNAs and transfected mRNA for selection
  • Increased titers by eliminating translational overhead of resistance genes
  • Selection processes include: magnetic isolation and depletion, FACs and death
Nathan Lewis

Protecting CHO cells from infection by adventitious agents

  • We have studied the molecular response of CHO cells to several viruses that have shut down production of biotherapeutics in various companies
  • We demonstrate that CHO cells can be more resistant to infection using a few different bioprocess treatments
  • A deep analysis of the infection and treatments highlights opportunities to engineer virus resistant cells
Elmira Jalilian

Generation of iPS-derived Endothelial Cells and its application in stem cell tissue engineering approaches

  • Differentiation of iPS-derived Endothelial Cells in vitro using 3D culture system.
  • Characterisation of in vitro generated cells using Next Generation Sequencing.  
  • Developing novel hybrid materials and 3D bioprinting technology that can control endothelial cell function in pre-vascularised muscle constructs.
Winnie Yeung

Round Tables

Roundtable 1: Cell culture medium- challenges and Solutions
  • Teresa Safly, Area Business Manager, Beckton Dickinson
  • Jennifer Gelman, Marketing Communication Manager, BD

Roundtable 2: Post translational regulation-The use of mRNA and Non Coding-RNA for cell line selection

  • Ted Eveleth,CEO, HocusLocus

Roundtable 3: Holistic statistical tools-  advancing biomanufacturing.

  • Martin Kane, Former Director of Process Statistics, GlaxoSmithKline

Roundtable 4: Continuous process- High density cell culture technologies and challenges

  • Winnie Yeung, Senior Research Associate II, Biologics Development- Cell Culture, Gilead Sciences
Winnie Yeung, Assoicate Scientist I, Gilead Sciences Ltd
Jennifer Gelman, Marketing Communication Manager, BD
Teresa Safly, Area Business Manager, BD Biosciences
Winnie Yeung, Assoicate Scientist I, Gilead Sciences Ltd


Process optimisation

Sung-Hye Grieco

Streamlining cell culture process development based on project needs

  • Developing project strategy and goals to facilitate collaboration
  • Examples of cross-functional data sharing to drive optimal process choice
  • Using DoE studies to build database to inform future process development
Matthew Stauffer

Bioreactor Process Improvements in a Perfusion-based Process

  • Process strategies to overcome the limitations of conventional batch cultivations
  • GMP compliant cultivation platforms
  • Developing continuous systems to increase the efficiency of production
Matthew Stauffer, Process Engineer II, Sanofi - Genzyme
Nobel Vale

Progress towards continuous upstream process monitoring using Raman spectroscopy

  • Taking advantage of real-time monitoring to learn more about your process and detect changes
  • Building methods to monitor nutrients and metabolites in bioprocessing
  • Utilizing Raman in a perfusion system to closely monitor dynamic changes in cell growth and nutrient consumption
Dr Alessandro Butte

Holistic approaches to process development: digitalization in process development

  • Statistical methods and multivariate decision tools
  • Machine learning and hybrid models
  • Process control using online monitoring and supervisory control
  • Quality prediction

Networking Coffee and Speed Networking

Joe Lattari

Dissolved carbon dioxide (DCO2) as a critical process parameter in upstream bioprocessing

  • DCO2 is a critical parameter from a quality and performance perspective, impacting productivity, growth rates, and product quality.
  • DCO2 concentration directly impacts key metabolic pathways and intracellular and extracellular pH
  • Inline DCO2 measurement can be critical for process control and optimization, scale-up and scale-down models, and process understanding
Hooman Hefzi

Elimination of lactate production in CHO cells

  • Complete knockout of lactate dehydrogenase
  • Drastic decrease in required base addition for pH stability in culture
  • Knockout cells exhibit prolonged growth in fed-batch culture
Khoi Thai

Implementation of Single Use Technologies at Commercial Scale

  • Single-use technologies (SUTs) are being rapidly adopted by the biopharmaceutical industry.
  • Overview of single-use technology - where and why SUT is used.
  • Challenges for implementation of single use for cell culture operations at commercial scale - what are the gaps?


last published: 11/May/17 14:45 GMT