Brandon Ruotolo | Professor
University of Michigan

Brandon Ruotolo, Professor, University of Michigan

Appearances:



Festival of Biologics Day 3 @ 09:00

Chair's opening remarks

Festival of Biologics Day 3 @ 12:10

Collision Induced Unfolding: Rapid, Sensitive, Information Rich Stability Measurements for Biotherapeutic Discovery and Development

The next generation of medicines will rely heavily upon our ability to quickly assess the structures and stabilities of protein-based biotherapeutics, including degradation products, engineered constructs, and antibody-antigen complexes.  Such endeavours are both slow and challenging using standard biophysical tools.  In this presentation, I discuss recent developments surrounding collision induced unfolding (CIU) methods that aim to bridge this technology gap. CIU uses ion mobility-mass spectrometry (IM-MS) to measure the stability and unfolding pathways of gas-phase proteins, without the need for covalent labels or tagging, and consuming 10-100 times less sample than almost any other label-free technology.  Recent developments in high-throughput CIU screening methods, their ability to track alterations in biomolecular structure as a function of stress, and software developments that seek to enhance CIU information content will be discussed. 

last published: 27/Mar/24 15:25 GMT

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